Beneficial effect of total flavonoids of Chrysanthemum indicum on adjuvant arthritis by induction of apoptosis of synovial fibroblasts

Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(23):2838-41
Authors: Xie XF, Li J, Chen Z, Hu CM, Chen WW

OBJECTIVE: To investigate the effect on the extract of total flavonoids of Chrysanthemum indicum (TFC) on adjuvant arthritis synovial cells. METHOD: SD rats were divided randomly into six groups including normal, model, TFC (84, 168, 336 mg x kg(-1)) and control drug Tripterygium glycosides (30 mg x kg(-1)) groups. Adjuvant arthritis rat model was induced by a single intradermal injection of 0.1 mL of the complete Freund’s adjuvant into the right hind feet pads of the SD rats. The proliferation of synoviocyte was measured by MT; The apoptosis rates of synovial cells were evaluated using TUNEL and FCM analysis. RESULT: TFC resulted in a dose-dependent way in inhibiting the proliferation of synovial and inducing the apoptosis of synovium and synoviocytes in vivo. CONCLUSION: TFC can inhibit proliferation and induce apoptosis in synovial cells, and exert therapeutical effect on rheumatoid arthritis.

PMID: 19260326 [PubMed – indexed for MEDLINE]